Two-phase unfolding pathway of ribonuclease A during denaturation induced by dithiothreitol
نویسندگان
چکیده
منابع مشابه
Two-phase unfolding pathway of ribonuclease A during denaturation induced by dithiothreitol.
The dynamics of the unfolding process of bovine pancreatic ribonuclease A (RNase A) unfolded by dithiothreitol (DTT) at a low concentration of 1:30 were investigated in alkaline phosphate-buffered saline solutions at 303K and 313K by using proton nuclear magnetic resonance ((1)H NMR) spectra. Three NMR spectral parameters including Shannon entropy, mutual information, and correlation coefficien...
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Inactivation of pancreatic RNAase A occurs in guanidinium chloride (GdmCl) at low concentrations before the unfolding of the molecule as a whole can be detected [Liu and Tsou (1987) Biochim. Biophys. Acta 916, 455-464]. We have now shown that the rate of digestion of the RNAase molecule by either trypsin or proteinase K increases significantly at low concentrations of GdmCl where the enzyme is ...
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Studies of the denaturation of proteins indicate certain characteristics of the forces which normally constrain them in their native chain conformations. In the case of bovine pancreatic ribonuclease, the description of the noncovalent bonds involved has taken on heightened interest following the recent report by White (1) that the integrity of the 4 disulfide bonds does not appear to be prereq...
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چکیده پلاتین، فلزی نجیب، پایدار و گران قیمت با خاصیت کاتالیزوری زیاد است که کاربرد های صنعتی فراوانی دارد. کمپلکس های پلاتین(ii) به عنوان دارو های ضد سرطان شناخته شدند و در شیمی درمانی بیماران سرطانی کاربرد دارند. خاصیت کاتالیزوری و عملکرد گزینشی پلاتین مستقیماً به اندازه و- شکل ماده ی پلاتینی بستگی دارد. بعضی از نانو ذرات فلزی در سطح مشترک مایع- مایع سنتز شده اند، اما نانو ساختار های پلاتین ب...
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ژورنال
عنوان ژورنال: Protein Science
سال: 2001
ISSN: 0961-8368,1469-896X
DOI: 10.1110/ps.20801